解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Duchenne and Becker muscular dystrophy (DMD/BMD) are X-linked muscular dystrophies. The isolation of the defective gene in DMD/BMD has led to a better understanding of the disease process and has promoted studies regarding the application of molecular therapy. The purpose of this review is to present the progress made...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540212333
更新日期:2002-03-01 00:00:00
abstract::In protein and RNA macromolecules, only a limited number of different side-chain chemical groups are available to function as catalysts. The myriad of enzyme-catalyzed reactions results from the ability of most of these groups to function either as nucleophilic, electrophilic, or general acid-base catalysts, and the k...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540211468
更新日期:2002-02-01 00:00:00
abstract::To further understand the role of the three conserved Val residues in insulin, B12Val, B18Val, and A3Val, five insulin mutants-[A3Ser]insulin, [B12Thr]insulin, (desB30)[B12Ser]insulin, [B18Thr] insulin, and [B18Leu]insulin--were obtained by means of site-directed mutagenesis and their receptor-binding activities as we...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/152165401317291165
更新日期:2001-12-01 00:00:00
abstract::Uncoupling proteins (UCPs) are regulated H+ transporters and a subfamily of the mitochondrial carrier family. Whereas UCP1 in brown adipose tissue has a well-defined role in thermogenesis, the roles of other UCPs are still tentative, such as in control of immune response, oxygen radical formation, and insulin secretio...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540152845975
更新日期:2001-09-01 00:00:00
abstract::The multifaceted relationship between mitochondria and the rest of the cell is reviewed in the context of myocardial ischemia. Paradoxically, mitochondria can exacerbate the ischemic damage, especially at the onset of reperfusion. Indeed, the recovery of oxidative phosphorylation in the presence of an excessive energy...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540152846073
更新日期:2001-09-01 00:00:00
abstract::Increasing evidence suggests a role for intracellular reactive oxygen species (ROS) as mediators of normal and pathological signal transduction pathways. In particular, a growing list of recent reports have demonstrated a rapid and significant increases in intracellular ROS following growth factor or cytokine stimulat...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540252774694
更新日期:2001-07-01 00:00:00
abstract::Anthracyclines are a class of antitumor drugs widely used for the treatment of a variety of malignancy, including leukemias, lymphomas, sarcomas, and carcinomas. Different mechanisms have been proposed for anthracycline antitumor effects including free-radical generation, DNA intercalation/binding, activation of signa...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540252774829
更新日期:2001-07-01 00:00:00
abstract::Myosin light chain kinase (MLCK) is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. Although this mode of action is well-established, there are numerous reports of smooth muscle contraction that is not associate...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/152165401753366087
更新日期:2001-06-01 00:00:00
abstract::The immunosuppressant cyclosporin A (CsA)-sensitive nuclear factor of activated T cells 1 (NFAT1) has been known to be a transcriptional regulator of cytokine and viral genes during the immune response. By analyses of serial deletion, mutation, and heterologous promoter assay, we report here that the CsA-sensitive NFA...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/152165401753311807
更新日期:2001-04-01 00:00:00
abstract::The expression of the cytM gene, which encodes cytochrome cM in Synechocystis sp. PCC 6803, is induced under stress conditions such as low temperature. Results of spectrophotometric studies revealed that cytochrome cM was oxidized by light absorbed by photosystem I in cells that had acclimatized to a low temperature. ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540118716
更新日期:2001-02-01 00:00:00
abstract::Iron presents us with a paradox. Without it, cells simply cannot survive because iron is an essential cofactor for many enzymes in critical biochemical pathways. However, when iron is present in excess, it can be highly cytotoxic due to its propensity to catalyze the formation of reactive oxygen radicals. To cater for...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540120450
更新日期:2001-01-01 00:00:00
abstract::Our results demonstrate that open reading frame (ORF) YHR076w on chromosome VIII of Saccharomyces cerevisiae that was previously described as a hypothetical gene is expressed. This ORF is transcribed as an mRNA of approximately 1,100 nucleotides. A 41.2-kDa polypeptide and three others predicted to be modified forms o...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803741
更新日期:2000-12-01 00:00:00
abstract::The best known NADPH oxidase is that of phagocytes-neutrophils and monocytes. In these cells, the enzyme manufactures large quantities of O2- and other reactive oxidants that are used for the purpose of killing invading microorganisms. Recent studies, however, have suggested that a number of other tissues contain NADP...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/713803730
更新日期:2000-10-01 00:00:00
abstract::Combining two analytical techniques, HPLC and liquid secondary ion mass spectrometry, the orientation of liposomal membrane-bound melittin was analyzed through its trypsin-digested products. We found that trypsin can access all proteolytic sites of the membrane-bound melittin when the liposomes have no transmembrane p...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/152165400300001543
更新日期:2000-09-01 00:00:00
abstract::In Streptomyces fradiae, calcium ions induce alterations in intensity and specificity of the secondary metabolism and stimulate sporulation. Using in vivo labeling, we demonstrate that in S. fradiae phosphorylation of some proteins are also influenced by Ca2+ added exogenously. Calcium ions at physiological concentrat...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803699
更新日期:2000-08-01 00:00:00
abstract::Human ecto-ATPase (ecto-nucleoside triphosphate diphosphohydrolase 2 [eNTPDase2], also known as CD39L1) has been expressed and characterized in COS cells. It exhibits some unusual enzymology that is similar to a few members of this class of proteins but different from the majority of the family members. Hydrolysis of ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540050176584
更新日期:2000-07-01 00:00:00
abstract::Retinitis pigmentosa comprises a large and exceptionally heterogeneous group of hereditary disorders of the retina. As a result of an extensive investigation around the world, primary genetic lesions have been described in many genes. Some of these genes encode enzymes that are involved in the signal transduction path...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540050167007
更新日期:2000-06-01 00:00:00
abstract::Acid fibroblast growth factor (aFGF) binds to its cell-surface receptors in a heparin-dependent manner. In an attempt to define the aFGF recognition site on fibroblast growth factor receptor 1 (FGFR1), we developed a screening strategy for identifying FGF ligands that bind to the receptor-binding region of FGF. To ret...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540050167106
更新日期:2000-06-01 00:00:00
abstract::Pancreatic islet beta-cells are poised to generate metabolic messengers in the mitochondria that link glucose metabolism to insulin exocytosis. This is accomplished through the tight coupling of glycolysis to mitochondrial activation. The messenger molecules ATP and glutamate are produced after the metabolism of glyco...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/152165400410236
更新日期:2000-05-01 00:00:00
abstract::The effect of streptozotocin-induced diabetes on cholinesterases activities was studied in the retina and, for comparison, in other nervous and nonnervous tissues. Streptozotocin diabetes did not affect acetylcholinesterase activity in the retina but increased its activity in the cerebral cortex (100%) and in serum (5...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540050033140
更新日期:2000-04-01 00:00:00
abstract::This review describes recent progress in the field of fibroblast growth factor receptors (FGFRs) with an emphasis on the role of FGFR mutants in skeletal malformations. This family of four receptors contains the most frequent germline mutations in humans. More than 75 mutations have been recorded, which account for mo...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/713803609
更新日期:2000-03-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV or HHV-8) is linked to the etiopathogenesis of Kaposi's sarcoma, a plasma-blastic variant of Castleman's disease and primary effusion lymphoma. KSHV is related to a number of non-human primate viruses. Only a limited number of KSHV proteins are expressed in tumor cells. He...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540050022395
更新日期:2000-02-01 00:00:00
abstract::In this study, two different epitope tags (HA, c-myc) were introduced near the N terminus of the yeast PMA1 H(+)-ATPase. The resulting proteins were indistinguishable from the wild-type ATPase in their ability to travel through the secretory pathway, as judged by quantitative immunoblotting of isolated secretory vesic...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540050022494
更新日期:2000-02-01 00:00:00
abstract::We previously found a novel glycosylphosphatidylinositol (GPI)-anchored glycoprotein designated GPI-80 that modulates complement receptor 3 integrin-dependent adhesion and in vitro transendothelial migration of neutrophils. In this study, we show that antibody-mediated cross-linking of GPI-80 led to rapid tyrosine pho...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803588
更新日期:2000-01-01 00:00:00
abstract::Alkyl-dihydroxyacetonephosphate synthase (alkyl-DHAP synthase) is a peroxisomal enzyme that plays a key role in ether phospholipid biosynthesis. To determine the turnover of alkyl-DHAP synthase in several peroxisomal disorders, pulse-chase experiments were performed. In control fibroblasts, mature alkyl-DHAP synthase ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803571
更新日期:1999-12-01 00:00:00
abstract::Women with breast cancer show altered blood glucose compartmentation compared with healthy women, with lower concentrations in plasma and similar concentrations in blood cells. The goal of this paper was to study whether this pattern was the result of changes in the erythrocyte glucose transporter and, if so, to asses...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803558
更新日期:1999-11-01 00:00:00
abstract::To establish possible cancerous aggressiveness between the metabolism of variously labeled [14C]glucose in the human breast invasive ductal carcinoma (IDC) tissues, we measured the rates of 14CO2 production from those tissues by using radiorespirometry, expressing the results as initial velocity (V) in nanomoles of 14...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803528
更新日期:1999-10-01 00:00:00
abstract::The acidic phospholipase A2 isoform from the spitting cobra Naja mossambica mossambica is activated irreversibly by treatment with a molar equivalent of oleoyl imidazolide. The kinetics of the chemical modification of the enzyme can also be monitored by measuring the large reduction of tryptophan fluorescence, which i...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803513
更新日期:1999-09-01 00:00:00
abstract::The sodA gene coding for manganese superoxide dismutase (Mn-SOD) from the marine microorganism Vibrio parahaemolyticus was cloned, sequenced, and overexpressed in Escherichia coli by use of the pET20b (+) expression vector. The full-length gene consisted of a 588-bp open reading frame and encoded a polypeptide of 196 ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803521
更新日期:1999-09-01 00:00:00
abstract::Recent structural studies of proteins mediating membrane fusion reveal intriguing similarities between diverse viral and mammalian systems. Particularly striking is the close similarity between the transmembrane envelope glycoproteins from the retrovirus HTLV-1 and the filovirus Ebola. These similarities suggest simil...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/713803503
更新日期:1999-08-01 00:00:00
abstract::To explore the oxygen response regulators involved in thiol peroxidase gene (tpx) expression in Escherichia coli, we constructed a single-copy tpx-lacZ operon fusion and monitored tpx-lacZ expression in various genetic backgrounds. Expression of the tpx-lacZ fusion was increased 4-fold by aerobic growth. Anaerobic exp...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803496
更新日期:1999-08-01 00:00:00
abstract::Although proteins and nucleic acids consist of different chemical components, proteins can mimic structures and possibly also functions of nucleic acids. Recently, structural mimicry was observed between two elongation factors in bacterial protein biosynthesis leading to the introduction of the concept of macromolecul...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/713803471
更新日期:1999-07-01 00:00:00
abstract::In tsRSV-infected NRK (tsNRK) cells, pp60(v-src) reactivation by temperature-shift from a nonpermissive temperature, 39 C, to a permissive one, 32 degrees C, induced the production of inositol phosphates (IPt) and phosphatidylethanol (PEt). This was accompanied by an increase in membrane-associated protein kinase C (P...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/713803467
更新日期:1999-07-01 00:00:00